Open AccessCharacterization of Anti- Salmonella enterica Serotype Typhi Antibody Responses in Bacteremic Bangladeshi Patients by an Immunoaffinity Proteomics-Based Technology
Author(s) -
Richelle C. Charles,
Alaullah Sheikh,
Bryan Krastins,
Jason B. Harris,
M. Saruar Bhuiyan,
Regina C. LaRocque,
Tanya Logvinenko,
David Sarracino,
Indira T. Kudva,
Jana Eisenstein,
Michael J. Podolsky,
Anuj Kalsy,
W. Abdullah Brooks,
Albrecht Ludwig,
Manohar John,
Stephen B. Calderwood,
Firdausi Qadri,
Edward T. Ryan
Publication year - 2010
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00104-10
Subject(s) - typhoid fever , salmonella typhi , serotype , antibody , salmonella enterica , microbiology and biotechnology , biology , antigen , virology , salmonella , proteomics , bacteremia , immunology , bacteria , escherichia coli , antibiotics , biochemistry , genetics , gene
Salmonella enterica serotype Typhi is the cause of typhoid fever and a human-restricted pathogen. Currently available typhoid vaccines provide 50 to 90% protection for 2 to 5 years, and available practical diagnostic assays to identify individuals with typhoid fever lack sensitivity and/or specificity. Identifying immunogenicS . Typhi antigens expressed during human infection could lead to improved diagnostic assays and vaccines. Here we describe a platformi mmunoaffinityp roteomics-basedt echnology (IPT) that involves the use of columns charged with IgG, IgM, or IgA antibody fractions recovered from humans bacteremic withS . Typhi to captureS . Typhi proteins that were subsequently identified by mass spectrometry. This screening tool identifies immunogenic proteins recognized by antibodies from infected hosts. Using this technology and the plasma of patients withS . Typhi bacteremia in Bangladesh, we identified 57 proteins ofS. Typhi, including proteins known to be immunogenic (PagC, HlyE, OmpA, and GroEL) and a number of proteins present in the human-restricted serotypesS . Typhi andS . Paratyphi A but rarely found in broader-host-rangeSalmonella spp. (HlyE, CdtB, PltA, and STY1364). We categorized identified proteins into a number of major groupings, including those involved in energy metabolism, protein synthesis, iron homeostasis, and biosynthetic and metabolic functions and those predicted to localize to the outer membrane. We assessed systemic and mucosal anti-HlyE responses inS . Typhi-infected patients and detected anti-HlyE responses at the time of clinical presentation in patients but not in controls. These findings could assist in the development of improved diagnostic assays.