Open AccessBlocking Effect of a Monoclonal Antibody against Recombinant Pvs25 on Sporozoite Development in Anopheles sinensis
Author(s) -
Sung-Ung Moon,
Hyung-Hwan Kim,
TongSoo Kim,
Kyung Il Choi,
Chang-Mi Oh,
Yong-Joo Ahn,
Seo-Kyoung Hwang,
Youngjoo Sohn,
E-Hyun Shin,
Hyuck Kim,
Hyeong-Woo Lee
Publication year - 2010
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00101-10
Subject(s) - recombinant dna , virology , monoclonal antibody , vector (molecular biology) , biology , anopheles sinensis , antibody , malaria vaccine , malaria , microbiology and biotechnology , antigen , titer , anopheles , gene , plasmodium falciparum , virus , immunology , genetics
To develop a vaccine to block the transmission of vivax malaria, the gene encoding the ookinete surface protein Pvs25 was cloned from a Korean malaria patient. ThePvs25 gene was 660 bp long, encoding 219 amino acids. It was subcloned into the expression vector pQE30 and expressed inEscherichia coli . The expressed recombinant protein, named rPvs25, showed a molecular mass of approximately 25 kDa by SDS-PAGE analysis. An anti-rPvs25 monoclonal antibody produced in BALB/c mice was able to inhibit sporozoite development in the mosquitoAnopheles sinensis , which is known as the malaria transmission vector in the Republic of Korea. In addition, rPvs25 produced a relatively high antibody titer in BALB/c mice that lasted for more than 6 months. Based on these results, we suggest that recombinant Pvs25 could be a useful antigen in the development of a vaccine to prevent local malaria transmission in the Republic of Korea.