Protective Efficacy of the Conserved NP, PB1, and M1 Proteins as Immunogens in DNA- and Vaccinia Virus-Based Universal Influenza A Virus Vaccines in Mice | Zendy

Wenling Wang | Zendy; Renqing Li | Zendy; Yao Deng | Zendy; Ning Lü | Zendy; Hong Chen | Zendy; Xin Meng | Zendy; Wen Wang | Zendy; Xiuping Wang | Zendy; Kexia Yan | Zendy; Xiangrong Qi | Zendy; Xiangmin Zhang | Zendy; Wei Xin | Zendy; Zhenhua Lü | Zendy; Xueren Li | Zendy; Tao Bian | Zendy; Yingying Gao | Zendy; Wenjie Tan | Zendy; Li Ruan | Zendy

Open Access

Protective Efficacy of the Conserved NP, PB1, and M1 Proteins as Immunogens in DNA- and Vaccinia Virus-Based Universal Influenza A Virus Vaccines in Mice

Author(s) -

Wenling Wang,

Renqing Li,

Yao Deng,

Ning Lü,

Hong Chen,

Xin Meng,

Wen Wang,

Xiuping Wang,

Kexia Yan,

Xiangrong Qi,

Xiangmin Zhang,

Wei Xin,

Zhenhua Lü,

Xueren Li,

Tao Bian,

Yingying Gao,

Wenjie Tan,

Li Ruan

Publication year - 2015

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00091-15

Subject(s) - virology , nucleoprotein , vaccinia , hemagglutinin (influenza) , antigenic drift , biology , neuraminidase , virus , influenza a virus , dna vaccination , viral matrix protein , heterologous , h5n1 genetic structure , immunogenicity , influenza vaccine , antigen , vaccination , immunization , recombinant dna , medicine , immunology , gene , genetics , infectious disease (medical specialty) , disease , covid-19 , pathology

The conventional hemagglutinin (HA)- and neuraminidase (NA)-based influenza vaccines need to be updated most years and are ineffective if the glycoprotein HA of the vaccine strains is a mismatch with that of the epidemic strain. Universal vaccines targeting conserved viral components might provide cross-protection and thus complement and improve conventional vaccines. In this study, we generated DNA plasmids and recombinant vaccinia viruses expressing the conserved proteins nucleoprotein (NP), polymerase basic 1 (PB1), and matrix 1 (M1) from influenza virus strain A/Beijing/30/95 (H3N2). BALB/c mice were immunized intramuscularly with a single vaccine based on NP, PB1, or M1 alone or a combination vaccine based on all three antigens and were then challenged with lethal doses of the heterologous influenza virus strain A/PR/8/34 (H1N1). Vaccines based on NP, PB1, and M1 provided complete or partial protection against challenge with 1.7 50% lethal dose (LD50 ) of PR8 in mice. Of the three antigens, NP-based vaccines induced protection against 5 LD50 and 10 LD50 and thus exhibited the greatest protective effect. Universal influenza vaccines based on the combination of NP, PB1, and M1 induced a strong immune response and thus might be an alternative approach to addressing future influenza virus pandemics.

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