Preparation of Monoclonal Antibodies for Detection and Identification of Francisella tularensis | Zendy

Akitoyo Hotta | Zendy; Akihiko Uda | Zendy; Osamu Fujita | Zendy; Kiyoshi Tanabayashi | Zendy; Akio Yamada | Zendy

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Preparation of Monoclonal Antibodies for Detection and Identification of Francisella tularensis

Author(s) -

Akitoyo Hotta,

Akihiko Uda,

Osamu Fujita,

Kiyoshi Tanabayashi,

Akio Yamada

Publication year - 2006

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00057-06

Subject(s) - francisella tularensis , monoclonal antibody , agglutination (biology) , microbiology and biotechnology , biology , tularemia , virology , antigen , antibody , virulence , biochemistry , immunology , gene

Monoclonal antibodies (MAbs) againstFrancisella tularensis were obtained. Three MAbs specifically reacted withF. tularensis , while four MAbs reacted with other members of the genusFrancisella as well. Fluorescent isothiocyanate-conjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinatedF. tularensis antigen in the agglutination tests. These MAbs should improve methods for detection and identification ofF. tularensis .

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