Serological Responses to Experimental Norwalk Virus Infection Measured Using a Quantitative Duplex Time-Resolved Fluorescence Immunoassay | Zendy

Owen Kavanagh | Zendy; Mary K. Estes | Zendy; Amanda Reeck | Zendy; Ravikiran M. Raju | Zendy; Antone R. Opekun | Zendy; Mark A. Gilger | Zendy; David Y. Graham | Zendy; Robert L. Atmar | Zendy

Open Access

Serological Responses to Experimental Norwalk Virus Infection Measured Using a Quantitative Duplex Time-Resolved Fluorescence Immunoassay

Author(s) -

Owen Kavanagh,

Mary K. Estes,

Amanda Reeck,

Ravikiran M. Raju,

Antone R. Opekun,

Mark A. Gilger,

David Y. Graham,

Robert L. Atmar

Publication year - 2011

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00039-11

Subject(s) - norwalk virus , immunoassay , seroconversion , serology , antibody , virology , fluorescence , duplex (building) , virus , microbiology and biotechnology , chemistry , biology , immunology , norovirus , biochemistry , dna , physics , quantum mechanics

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

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