Open AccessUse of Protein-Specific Monoclonal Antibody-Based Latex Agglutination for Rapid Diagnosis of Burkholderia pseudomallei Infection in Patients with Community-Acquired Septicemia
Author(s) -
Pattama Ekpo,
Utane Rungpanich,
Supinya Pongsunk,
Pimjai Naigowit,
Vimon Petkanchanapong
Publication year - 2007
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00011-07
Subject(s) - burkholderia pseudomallei , latex fixation test , melioidosis , microbiology and biotechnology , burkholderia , monoclonal antibody , blood culture , direct agglutination test , agglutination (biology) , antibody , monoclonal , medicine , sepsis , immunology , biology , bacteria , serology , antibiotics , genetics
A latex agglutination test employing monoclonal antibody specific to a 30-kDa protein ofBurkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. The sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.