Open AccessClonal Analysis of the Human B-Cell Repertoire Using a Heteromobility Assay
Author(s) -
Deepanker Tewari
Publication year - 2000
Publication title -
clinical and diagnostic laboratory immunology
Language(s) - English
Resource type - Journals
eISSN - 1098-6588
pISSN - 1071-412X
DOI - 10.1128/cdli.7.3.507-509.2000
Subject(s) - microbiology and biotechnology , antibody , dna , biology , duplex (building) , cell , computational biology , genetics
A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.