Open AccessIdentification of Potentially DiagnosticLeishmania braziliensisAntigens in Human Cutaneous Leishmaniasis by Immunoblot Analysis
Author(s) -
Maria Edileuza Felinto de Brito,
Mitzi G. Mendonça,
Yara M. Gomes,
M. L. Jardim,
Frederico G. C. Abath
Publication year - 2000
Publication title -
clinical and diagnostic laboratory immunology
Language(s) - English
Resource type - Journals
eISSN - 1098-6588
pISSN - 1071-412X
DOI - 10.1128/cdli.7.2.318-321.2000
Subject(s) - cutaneous leishmaniasis , antigen , leishmania braziliensis , leishmaniasis , leishmania , immunofluorescence , indirect immunofluorescence , biology , antibody , immunology , virology , microbiology and biotechnology , parasite hosting , world wide web , computer science
The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens ofLeishmania braziliensis . The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be significantly more sensitive and specific than indirect immunofluorescence and enzyme-linked immunosorbent assay.