Open AccessClustering of Clinical Strains of Helicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis
Author(s) -
Helena Enroth,
Thomas Åkerlund,
Anna Sillén,
Lars Engstrand
Publication year - 2000
Publication title -
clinical and diagnostic laboratory immunology
Language(s) - English
Resource type - Journals
eISSN - 1098-6588
pISSN - 1071-412X
DOI - 10.1128/cdli.7.2.301-306.2000
Subject(s) - helicobacter pylori , dendrogram , biology , gel electrophoresis , strain (injury) , polyacrylamide gel electrophoresis , two dimensional gel electrophoresis , genome , genetics , microbiology and biotechnology , gene , proteomics , medicine , biochemistry , enzyme , genetic diversity , population , environmental health , anatomy
Strain variations ofHelicobacter pylori have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whetherH. pylori expresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE).H. pylori strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in theH. pylori genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation thatH. pylori exhibits.