Open AccessInfluence of different conditions on kinetics of tumor necrosis factor alpha release by peripheral blood mononuclear cells after stimulation with Cryptococcus neoformans: a possible explanation for different results
Author(s) -
Wendy Chaka,
A. F. M. Verheul,
Andy I. M. Hoepelman
Publication year - 1997
Publication title -
clinical and diagnostic laboratory immunology
Language(s) - English
Resource type - Journals
eISSN - 1098-6588
pISSN - 1071-412X
DOI - 10.1128/cdli.4.6.792-794.1997
Subject(s) - peripheral blood mononuclear cell , cryptococcus neoformans , tumor necrosis factor alpha , stimulation , kinetics , immunology , peripheral blood , cryptococcus , chemistry , alpha (finance) , microbiology and biotechnology , biology , medicine , in vitro , biochemistry , endocrinology , physics , construct validity , nursing , patient satisfaction , quantum mechanics
In available literature, different kinetics for tumor necrosis factor alpha (TNF-alpha) release by peripheral blood mononuclear cells are reported upon stimulation with Cryptococcus neoformans. Results in this study showed that shaking cells gives faster kinetics of TNF-alpha release while large working volumes give lower TNF-alpha concentrations. Different experimental conditions thus influence kinetics of TNF-alpha release by phagocytes.