Open AccessCytomegalovirus glycoprotein B-specific antibody analysis using electrochemiluminescence detection-based techniques
Author(s) -
Mats Ohlin,
Michel Silvestri,
ViviAnne Sundqvist,
Carl Borrebaeck
Publication year - 1997
Publication title -
clinical and diagnostic laboratory immunology
Language(s) - English
Resource type - Journals
eISSN - 1098-6588
pISSN - 1071-412X
DOI - 10.1128/cdli.4.1.107-111.1997
Subject(s) - electrochemiluminescence , glycoprotein , cytomegalovirus , monoclonal antibody , epitope , antibody , neutralization , chemistry , virology , microbiology and biotechnology , computational biology , biology , chromatography , virus , immunology , herpesviridae , detection limit , viral disease
An electrochemiluminescence technique was used to develop versatile and sensitive assay strategies for determination of seroreactivities against biologically important cytomegalovirus neutralization epitopes expressed on glycoprotein B. Indirect binding assays showed wide linear assay ranges and revealed that serum samples diluted in parallel with a monoclonal antibody-based standard, simplifying quantitative analytical assessments.