Open AccessA new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant
Author(s) -
Ellen M. Mascini,
M A Hazenberg,
Liesbeth Verhage,
Stig E. Holm,
J. Verhoef,
Hans van Dijk
Publication year - 1996
Publication title -
clinical and diagnostic laboratory immunology
Language(s) - English
Resource type - Journals
eISSN - 1098-6588
pISSN - 1071-412X
DOI - 10.1128/cdli.3.6.779-781.1996
Subject(s) - streptococcus pyogenes , exotoxin , microbiology and biotechnology , streptococcus , group a , streptococcal infections , pathogenesis , streptococcaceae , chemistry , biology , bacteria , medicine , toxin , immunology , staphylococcus aureus , antibiotics , genetics
An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (> or = 99%), and time.