Open AccessInterlaboratory Reliability of Microimmunofluorescence Test for Measurement of Chlamydia pneumoniae -Specific Immunoglobulin A and G Antibody Titers
Author(s) -
Alyson J. Littman,
Lisa A. Jackson,
Emily White,
Mark Thornquist,
Charlotte A. Gaydos,
Thomas L. Vaughan
Publication year - 2004
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cdli.11.3.615-617.2004
Subject(s) - chlamydia , chlamydophila pneumoniae , immunology , antibody , titer , chlamydiaceae , immunoglobulin g , antibody titer , immunoglobulin a , immunoglobulin m , chlamydiales , biology , medicine
To evaluate the reliability of Chlamydia pneumoniae-specific immunoglobulin G (IgG) and IgA antibody titers as measured by the microimmunofluorescence (MIF) test, we compared results from 392 individuals using a standard MIF protocol at two academic laboratories. The kappas for dichotomous titers (>/=16 versus <16) were 0.39 for IgA and 0.53 for IgG. Measurement error likely attenuates C. pneumoniae-disease associations; the magnitude of attenuation can be estimated from results of studies such as this one.