Open AccessLiquid Nitrogen Cryo-Impacting: a New Concept for Cell Disruption
Author(s) -
Richard A. Smucker,
Robert M. Pfister
Publication year - 1975
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.30.3.445-449.1975
Subject(s) - bacillus megaterium , dipicolinic acid , lactate dehydrogenase , liquid nitrogen , endospore , escherichia coli , nitrogen , chemistry , bacteria , breakage , biochemistry , microbiology and biotechnology , biology , materials science , enzyme , spore , organic chemistry , genetics , gene , composite material
High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage ofBacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-brokenEscherichia coli cells than in those from sonically treated and brokenE. coli cells.
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