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High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage ofBacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-brokenEscherichia coli cells than in those from sonically treated and brokenE. coli cells.

Liquid Nitrogen Cryo-Impacting: a New Concept for Cell Disruption