Aliphatic Hydrocarbons of Cladosporium resinae Cultured on Glucose, Glutamic Acid, and Hydrocarbons

The carbon source markedly influenced the qualitative and quantitative composition of cellular hydrocarbons inCladosporium resinae . Total lipid and hydrocarbon content was greater in cells grown onn -alkanes than in cells grown on glucose or glutamic acid. Glucose-grown cells contained a spectrum of aliphatic hydrocarbons from C7 to C36 ; pristane andn -hexadecane comprised 98% of the total. Cells grown on glutamic acid contained C7 to C23 hydrocarbons;n -tridecane,n -tetradecane,n -hexadecane, and pristane made up 74% of the total.n -Decane-grown cells yielded C8 to C32 compounds, andn -hexadecane (96%) was the major hydrocarbon. Cells grown on individualn -alkanes from C11 to C15 all contained C11 to C28 hydrocarbons, and cells grown onn -hexadecane contained C11 to C32 hydrocarbons. Inn -undecane-grown cells,n -hexadecane and pristane made up 92% of the total, but in cells grown on C12 to C16 n -alkanes the major cellular hydrocarbon was the one on which the cells were grown. This suggests that cells cultured onn -alkanes of C12 or longer accumulaten -alkanes prior to oxidizing them.