Open AccessIsolation of Group H Streptococcal Competence Factor
Author(s) -
Dennis Perry
Publication year - 1973
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.26.4.643-645.1973
Subject(s) - muramic acid , orcinol , chemistry , sephadex , fractionation , trypsin , glucosamine , chromatography , biochemistry , enzyme , peptidoglycan
Competence factor (CF) was purified 600-fold from culture filtrates of competent group H streptococcus, strain Challis, by fractionation on a carboxymethyl-Sephadex column. The preparation did not contain orcinol- or diphenylamine-positive material, and CF activity was destroyed by trypsin. An examination of its chemical composition revealed 10 amino acids, muramic acid, glucosamine, and glucose.
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