Rapid Method for Detection of Pseudomonas aeruginosa on MacConkey Agar Under Ultraviolet Light
Author(s) -
Michael H. Brodsky,
M. C. Nixon
Publication year - 1973
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.26.2.219-220.1973
Subject(s) - pseudomonas aeruginosa , macconkey agar , microbiology and biotechnology , agar , ultraviolet , ultraviolet light , agar plate , fluorescence , chemistry , biology , chromatography , bacteria , physics , optics , photochemistry , genetics
A simple screening technique for the detection ofPseudomonas aeruginosa colonies by their fluorescence on MacConkey agar under ultraviolet light is proposed. From 306 nonlactose fermenting cultures screened under the ultraviolet light, 108 fluorescent isolates were obtained. These were screened biochemically, with 103 (94.8%) being verified asP. aeruginosa . From the 198 nonfluorescing cultures, only one suspectedP. aeruginosa was isolated.
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