Improved Hemagglutination Inhibition Assay for Mammary Tumor Virus Antigen
Author(s) -
Ronald W. Gillette,
Diane Junker
Publication year - 1973
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.26.1.63-65.1973
Subject(s) - tannic acid , hemagglutination assay , antigen , formaldehyde , hemagglutination , chemistry , virology , virus , microbiology and biotechnology , biology , biochemistry , immunology , organic chemistry , titer
It is our experience that the use of sheep red blood cells pretreated with formaldehyde and pyruvaldehyde before sensitizing with viral antigen yields results that are comparable to that obtained by using the tannic acid method. The double aldehyde method provides the added advantage of being useful for assaying the viral antigen content in substances which contain high levels of interfering substances which would preclude the use of other assay methods.
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