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The isolation of an alkane-oxidizing strain ofPseudomonas oleovorans which maintains its viability at 5 C is described. This strain epoxidates 1-octene at a rate five times that of the parent strain. The most efficient substrates for induction of the epoxidase are C7 , C8 , and C9 , although C5 to C12 also serve as growth substrates and inducers. The greater rate may be attributed to an enhanced general stability of the cells as opposed to a modification of the enzyme system involved.

Octene Epoxidation by a Cold-Stable Alkane-Oxidizing Isolate of Pseudomonas oleovorans