Comparison of the Direct Agglutination and Indirect Hemagglutination Tests in the Determination of Blood Serum Titers to Escherichia coli Organisms
Author(s) -
I. A. Schipper,
C. Kelling,
H. Ebeltoft,
David A. Graves
Publication year - 1973
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.25.3.458-460.1973
Subject(s) - hemagglutination , agglutination (biology) , titer , microbiology and biotechnology , direct agglutination test , hemagglutination assay , hemagglutination tests , serology , latex fixation test , escherichia coli , antigen , biology , antibody , virology , immunology , biochemistry , gene
A comparison of the direct agglutination test and the indirect hemagglutination test for the detection of blood serum antibodies toEscherichia coli organisms indicated that these serological tests were comparable. In some instances the indirect hemagglutination test provided higher endpoint readings. Preparation of the antigens for the indirect hemagglutination test was more time consuming than for the direct agglutination test. Crude extract and purified polysaccharides were comparable as red blood cell sensitizing agents.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom