Open AccessTurbidimetric Assay of Staphylococcal Nuclease
Author(s) -
Alan K. Erickson,
R. H. Deibel
Publication year - 1973
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.25.3.337-341.1973
Subject(s) - nuclease , micrococcal nuclease , chemistry , chromatography , enzyme , quantitative analysis (chemistry) , biochemistry , dna , nucleosome , histone
A simplified turbidimetric procedure was developed to assay staphylococcal nuclease activity. The ease of performance and sensitivity to nanogram quantities enhance the utilization of the method for the quantitative or qualitative estimation of the enzyme. Unlike plating methods, the turbidimetric procedure affords the differentiation between heat-stable and heat-labile nuclease activity.
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