Demonstration of Rhodanese Activity in Polyacrylamide Gels | Zendy

John R. Murphy | Zendy; J. Frankenfeld | Zendy; Jay W. Calvert | Zendy; Richard C. Tilton | Zendy

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Demonstration of Rhodanese Activity in Polyacrylamide Gels

Author(s) -

John R. Murphy,

J. Frankenfeld,

Jay W. Calvert,

Richard C. Tilton

Publication year - 1972

Publication title -

applied microbiology

Language(s) - English

Resource type - Journals

ISSN - 0003-6919

DOI - 10.1128/am.24.2.283-285.1972

Subject(s) - polyacrylamide , rhodanese , incubation , staining , chemistry , sulfite , chromatography , polyacrylamide gel electrophoresis , strain (injury) , chromatium , biochemistry , biology , enzyme , polymer chemistry , photosynthesis , anatomy , genetics

Rhodanese activity from crude extracts ofThiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 × 10-7 moles of sulfite.

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