Nucleic Acid Enzyme Studies of Nonfermentative Gram-Negative Bacteria Using Thin-Layer Chromatography | Zendy

Marilyn M. Klein | Zendy; Donna J. Blazevic | Zendy

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Nucleic Acid Enzyme Studies of Nonfermentative Gram-Negative Bacteria Using Thin-Layer Chromatography

Author(s) -

Marilyn M. Klein,

Donna J. Blazevic

Publication year - 1972

Publication title -

applied microbiology

Language(s) - English

Resource type - Journals

ISSN - 0003-6919

DOI - 10.1128/am.23.2.276-280.1972

Subject(s) - bacteria , thin layer chromatography , pseudomonas , nucleic acid , enzyme , biochemistry , microbiology and biotechnology , biology , paper chromatography , gram , gram negative bacteria , nucleoside , chemistry , chromatography , escherichia coli , genetics , gene

A rapid and easy to perform procedure for determining the nucleic acid enzyme reactions of intact bacterial cells was developed. Overnight organism growth on triple sugar agar was tested for nucleoside phosphotransferase, nucleosidase, and nucleotidase activity. Reaction products were detected by means of thin-layer chromatography and fluorescence. Characteristic patterns were seen with certain strains of nonfermentative gram-negative bacteria, which indicated that these tests could aid in classification and identification of E0-1 group,Pseudomonas multivorans, P. cepacia, P. maltophilia , certain otherPseudomonas species, andHerellea vaginicola .

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