Open AccessInhibition of Nucleic Acid and Protein Synthesis in Mouse Spleen Cells In Vitro by Azathioprine
Author(s) -
Grace Chan,
Robert L. Stone
Publication year - 1970
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.20.6.910-912.1970
Subject(s) - nucleic acid , in vitro , uridine , spleen , thymidine , biochemistry , protein biosynthesis , azathioprine , dna synthesis , microbiology and biotechnology , dna , biosynthesis , leucine , rna , chemistry , biology , amino acid , immunology , enzyme , gene , medicine , disease
The effect of azathioprine on macromolecular biosynthesis was studied in mouse spleen cells cultured in vitro. The rate of incorporation of3 H-thymidine,3 H-uridine, and14 C-leucine into acid-insoluble material was used to measure deoxyribonucleic acid, ribonucleic acid, and protein synthesis. Results indicate that azathioprine inhibited nucleic acid and protein synthesis at levels which did not decrease cell viability.
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