Automated, Quantitative Microhemagglutination Assay for Treponema pallidum Antibodies | Zendy

Patricia M. Cox | Zendy; Leslie C. Logan | Zendy; Leslie C. Norins | Zendy

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Automated, Quantitative Microhemagglutination Assay for Treponema pallidum Antibodies

Author(s) -

Patricia M. Cox,

Leslie C. Logan,

Leslie C. Norins

Publication year - 1969

Publication title -

applied microbiology

Language(s) - English

Resource type - Journals

ISSN - 0003-6919

DOI - 10.1128/am.18.3.485-489.1969

Subject(s) - treponema , serial dilution , antibody , microbiology and biotechnology , biology , bacteria , virology , syphilis , immunology , medicine , pathology , genetics , alternative medicine , human immunodeficiency virus (hiv)

An automated, quantitative microhemagglutination assay for antibodies toTreponema pallidum was developed by usingT. pallidum -sensitized erythrocytes and an automatic serial-dilution instrument. Reactivity was found in sera from 54 rabbits and 6 chimpanzees infected withT. pallidum. Reactivity was also found in sera from animals infected withT. pertenue, T. carateum , andT. cuniculi . No reactivity was found in sera from 75 normal rabbits or from 129 rabbits immunized with cultivatable treponemes or a variety of other bacteria. In approximately 3 min, 13 twofold serial dilutions of each of 8 preabsorbed sera and the addition of sensitized erythrocytes to each dilution were accomplished automatically. The automated assay can serve as a research tool in quantitating antibodies to pathogenic treponemes, and evaluation of its clinical usefulness seems warranted.

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