Bioassay for Hamycin and Amphotericin B in Serum and Other Biological Fluids | Zendy

Smith Shadomy | Zendy; J. A. McCay | Zendy; Stephen Schwartz | Zendy

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Bioassay for Hamycin and Amphotericin B in Serum and Other Biological Fluids

Author(s) -

Smith Shadomy,

J. A. McCay,

Stephen Schwartz

Publication year - 1969

Publication title -

applied microbiology

Language(s) - English

Resource type - Journals

ISSN - 0003-6919

DOI - 10.1128/am.17.4.497-503.1969

Subject(s) - bioassay , amphotericin b , paecilomyces , polyene , pharmacology , antifungal , biological fluids , medicine , chemistry , biology , microbiology and biotechnology , chromatography , biochemistry , genetics , botany

A bioassay suitable for measuring concentrations of the polyene antifungal agents hamycin and amphotericin B in biological fluids is described. By usingPaecilomyces varioti as the indicator organism, sensitivity of the bioassay was found to be in the range of 0.01 to 0.02 μg/ml. A linear dose-response curve was obtained with amphotericin B; the curve for hamycin was curvilinear. In a series of assays, hamycin serum levels in the range of 0.01 to 3.5 μg/ml were measured; with amphotericin B, serum levels in the range of 0.015 to 0.175 μg/ml were measured in patients receiving orthodox intravenous medication and as high as 9.0 μg/ml in one patient treated with extraordinarily high doses of the drug.

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