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Sodium nitrite alone has been shown to stimulate germination of PA 3679h spores. The process was accelerated by using increased concentrations of sodium nitrite, a lowp H, and a high temperature of incubation. At low concentrations of nitrite (0.01 to 0.2%), the delay of 36 to 48 hr occurred before germination commenced at 37 C. However, with 3.45% nitrite at 45 C andp H 6.0, most of the spores germinated within 1 hr. Atp H 7.0, the germination rate decreased markedly, and atp H 8.0 it was nil. The greatest acceleration in germination rate occurred near 60 C. Hydroxylamine was completely inhibitory to nitrite-induced germination. Sodium nitrite, in turn, inhibited germination byl -alanine, the degree of inhibition being influenced by nitrite concentration andp H.

Nitrite-induced Germination of Putrefactive Anaerobe 3679h Spores