Production of Clostridium pasteurianum in a Defined Medium | Zendy

K. Sargeant | Zendy; J. W. S. Ford | Zendy; V. M. C. Longyear | Zendy

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Production of Clostridium pasteurianum in a Defined Medium

Author(s) -

K. Sargeant,

J. W. S. Ford,

V. M. C. Longyear

Publication year - 1968

Publication title -

applied microbiology

Language(s) - English

Resource type - Journals

ISSN - 0003-6919

DOI - 10.1128/am.16.2.296-300.1968

Subject(s) - sucrose , liter , dry weight , ammonium , nitrogen , clostridium , yield (engineering) , chemistry , carbon fibers , food science , ferredoxin , zoology , biochemistry , biology , bacteria , materials science , agronomy , organic chemistry , metallurgy , genetics , composite number , composite material , enzyme , endocrinology

A method for the growth ofClostridium pasteurianum in a 140-liter (total capacity) stainless-steel vessel is described. By preventing thep H value from falling below 5.6, the growth of cultures was prolonged. Larger amounts of the carbon source (sucrose) and the nitrogen source (ammonium ion) were supplied and consumed, and cell yields of up to 5.56 g (dry weight) per liter were obtained. The highest cell yield previously reported was 1.7 g (dry weight) per liter obtained under nitrogen-fixing conditions in 500-ml cultures. The ferredoxin content of the cells was comparable with that obtained by earlier workers.

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