Pigmented Bacteriostatic Substances and Amino Acids Produced by Phlebopus sulphureus and Phlebopus lignicola

The pigmented bacteriostatic substances and ninhydrin-reactive compounds produced in culture by sporophores, stipes, and residual medium ofPhlebopus sulphureus andP. lignicola were studied chromatographically. Acetone extracts of both species demonstrated bacteriostatic activity againstBacillus subtilis andEscherichia coli , the maximal inhibition zone being 13 mm. The chloroform extract was inactive. Six compounds, mainly yellow, extracted in ethyl alcohol and separated by chromatography, were common to the sporophore ofP. sulphureus and the stipes ofP. lignicola . Eight amino acids, mainly sulfur-containing, were identified chiefly from the mature sporophore ofP. sulphureus .