Trypsinization of Animal Tissues for Cell Culture: Theoretical Considerations and Automatic Apparatus | Zendy

Gio Batta Gori | Zendy

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Trypsinization of Animal Tissues for Cell Culture: Theoretical Considerations and Automatic Apparatus

Author(s) -

Gio Batta Gori

Publication year - 1964

Publication title -

applied microbiology

Language(s) - English

Resource type - Journals

ISSN - 0003-6919

DOI - 10.1128/am.12.2.115-121.1964

Subject(s) - trypsinization , volume (thermodynamics) , process (computing) , chemistry , chromatography , biology , computer science , biochemistry , thermodynamics , trypsin , physics , enzyme , operating system

The theoretical background and the experimental feasibility of an automatic control for the continuous trypsinization process are discussed. An automatic apparatus is described with experimental evidence that the optimal mean residence time of monkey kidney cells in the trypsinization flask is between 5 and 8 min, in a volume of fluid approximately ten times that of the tissue processed. A temperature of 36 C and a pH of 7.8 provide optimal conditions for cell viability.

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