Open AccessMolecular Phylogeny of Anabaena circinalis and Its Identification in Environmental Samples by PCR
Author(s) -
Kim M. Fergusson,
Christopher P. Saint
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.9.4145-4148.2000
Subject(s) - biology , saxitoxin , polymerase chain reaction , microbiology and biotechnology , anabaena , phylogenetics , phylogenetic tree , dna sequencing , sequence analysis , toxin , gene , genetics , cyanobacteria , bacteria
Although the cyanobacteriumAnabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification ofA. circinalis in a mixed population is complicated due to limited morphological differences betweenAnabaena species. Sequence analysis of the DNA-dependent RNA polymerase (rpoC1 ) gene from 24Anabaena isolates, including 12 designatedA. circinalis , permitted a phylogenetic analysis to be performed. In addition, anA. circinalis -specific PCR was developed and tested successfully on environmental samples.