Open AccessProduction and Characterization of Two Monoclonal Antibodies Specific for Plasmopara halstedii
Author(s) -
Sandrine Bouterige,
Raymond Robert,
JeanPhilippe Bouchara,
A. Marot-Leblond,
Valérie Molinéro,
Jean-Marcel Senet
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.8.3277-3282.2000
Subject(s) - downy mildew , biology , monoclonal antibody , epitope , antigen , helianthus annuus , plasmopara viticola , antibody , virology , microbiology and biotechnology , sunflower , botany , genetics , horticulture
Sunflower downy mildew, caused by the fungusPlasmopara halstedii , is a potentially devastating disease. We produced two monoclonal antibodies (MAbs) (12C9 and 18E2) by immunizing mice with a partially purified extract ofP. halstedii race 1. Both MAbs detected in enzyme-linked immunosorbent assay (ELISA) all races ofP. halstedii present in France. No cross-reactions were observed withPlasmopara viticola or with other fungi commonly associated with sunflowers. Both MAbs recognized the same three fungal antigens with molecular masses of 68, 140, and 192 kDa. However, the epitopes on the fungal antigens were distinct and repetitive. Seed homogenates from infected plants were incubated in wells coated with MAb 18E2. This resulted in the trapping ofP. halstedii antigens that were identified with biotinylated MAb 12C9. No reactions were seen with seed homogenates from healthy plants. Thus, our results suggest that these MAbs might be used to develop a sandwich ELISA detection system forP. halstedii in infected seeds.