Immunolocalization of Dinitrogenase Reductase Produced by Klebsiella pneumoniae in Association with Zea mays L | Zendy

Marisa K. Chelius | Zendy; Eric W. Triplett | Zendy

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Immunolocalization of Dinitrogenase Reductase Produced by Klebsiella pneumoniae in Association with Zea mays L

Author(s) -

Marisa K. Chelius,

Eric W. Triplett

Publication year - 2000

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.66.2.783-787.2000

Subject(s) - klebsiella pneumoniae , green fluorescent protein , bacteria , biology , klebsiella , colonization , nitrogenase , zea mays , microbiology and biotechnology , diazotroph , escherichia coli , nitrogen fixation , biochemistry , gene , agronomy , genetics

The endophytic lifestyle ofKlebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize byK. pneumoniae strains 2028 and 342. These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization. This activity was only apparent when bacteria were supplied with an exogenous carbon source. The results suggest that maize provides a suitable habitat forK. pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.

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