Open AccessRapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition
Author(s) -
Robert I. Griffiths,
Andrew S. Whiteley,
Anthony G. O'Donnell,
Mark Bailey
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.12.5488-5491.2000
Subject(s) - ribosomal rna , temperature gradient gel electrophoresis , ribosomal dna , biology , dna , microbial population biology , dna extraction , nucleic acid , 18s ribosomal rna , 16s ribosomal rna , microbiology and biotechnology , polymerase chain reaction , bacteria , genetics , gene , phylogenetics
A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.