Open AccessCharacterization of Cryptosporidium parvum by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
Author(s) -
Matthew L. Magnuson,
James H. Owens,
Catherine A. Kelty
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.11.4720-4724.2000
Subject(s) - cryptosporidium parvum , mass spectrometry , matrix assisted laser desorption/ionization , chemistry , cryptosporidium , biology , matrix (chemical analysis) , chromatography , analytical chemistry (journal) , microbiology and biotechnology , desorption , feces , organic chemistry , adsorption
Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawedCryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns forC. parvum were distinguishable from those obtained forCryptosporidium muris . One spectral marker appears specific for the genus, while others appear specific at the species level. Three differentC. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.