Open AccessEvaluation of Immunomagnetic Separation for Recovery of Infectious Cryptosporidium parvum Oocysts from Environmental Samples
Author(s) -
Paul A. Rochelle,
Ricardo León,
Margaret Johnson,
Mic H. Stewart,
Roy L. Wolfe
Publication year - 1999
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.65.2.841-845.1999
Subject(s) - cryptosporidium parvum , immunomagnetic separation , cryptosporidium , biology , microbiology and biotechnology , apicomplexa , chromatography , virology , feces , chemistry , protozoal disease , immunology , malaria
Two commercial immunomagnetic separation (IMS) kits forCryptosporidium were compared for recovery of oocysts from environmental samples. Oocyst recovery efficiencies with the Dynal and Crypto-Scan kits ranged from 62 to 100% and 34 to 74%, respectively, for seeded environmental water concentrates (turbidity of 210 to 11,480 nephelometric turbidity units). Recovery efficiencies were dependent on the mechanism of agitation during the magnetic capture procedure. An assay combining in vitro cell culture and reverse transcriptase PCR demonstrated that oocysts recovered by IMS retained their infectivity.
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