A new membrane filtration medium for simultaneous detection and enumeration of Escherichia coli and total coliforms | Zendy

Malcolm A. Grant | Zendy

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A new membrane filtration medium for simultaneous detection and enumeration of Escherichia coli and total coliforms

Author(s) -

Malcolm A. Grant

Publication year - 1997

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.63.9.3526-3530.1997

Subject(s) - escherichia coli , enumeration , filtration (mathematics) , agar , enterobacteriaceae , microbiology and biotechnology , biology , nutrient agar , bacteria , chromatography , food science , chemistry , biochemistry , mathematics , statistics , genetics , combinatorics , gene

Recovery of total coliforms and Escherichia coli on a new membrane filtration (MF) medium was evaluated with 25 water samples from seven states. Testing of the new medium, m-ColiBlue24 broth, was conducted according to a U.S. Environmental Protection Agency protocol. For comparison, this same protocol was used to measure recovery of total coliforms and E. coli with two standard MF media, m-Endo broth and mTEC broth. E. coli recovery on the new medium was also compared to recovery on nutrient agar supplemented with 4-methylumbelliferyl-beta-D-glucuronide. Comparison of specificity, sensitivity, false positive error, undetected target error, and overall agreement indicated E. coli recovery on m-ColiBlue24 was superior to recovery on mTEC for all five parameters. Recovery of total coliforms on the new medium was comparable to recovery on m-Endo.

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