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Comparison of commercially available kits with standard methods for detection of Salmonella strains in foods
Author(s) -
Kazunari Hanai,
Mikio Satake,
Hirotaka Nakanishi,
Kasthuri Venkateswaran
Publication year - 1997
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.63.2.775-778.1997
Subject(s) - salmonella , serotype , brilliant green , food and drug administration , agar , isolation (microbiology) , immunomagnetic separation , microbiology and biotechnology , xylose , food science , biology , food microbiology , chemistry , bacteria , fermentation , genetics , organic chemistry , pharmacology
Six commercial kits were compared with the U.S. Food and Drug Administration (USFDA) method and the Japanese standard method for Salmonella isolation in foods. When only Salmonella serovars were tested, many of the methods performed well; however, when foods were artificially inoculated, only the USFDA method and immunomagnetic separation coupled with the xylose-lysine-brilliant green agar method (MS-XLBG) could positively detect Salmonella serovars. All seven wild-type Salmonella serovars were detected by the USFDA method, and the MS-XLBG method detected salmonellae from six samples.

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