Characterization of an 8.7-kilobase thiostrepton resistance-encoding plasmid (pGIF3) of Streptomyces incarnatus | Zendy

Halina Z Malina | Zendy; Malka Robert-Géro | Zendy

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Characterization of an 8.7-kilobase thiostrepton resistance-encoding plasmid (pGIF3) of Streptomyces incarnatus

Author(s) -

Halina Z Malina,

Malka Robert-Géro

Publication year - 1992

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.58.3.895-899.1992

Subject(s) - thiostrepton , plasmid , replicon , biology , streptomyces , pbr322 , escherichia coli , genetics , cloning vector , gene , southern blot , molecular cloning , microbiology and biotechnology , bacteria , gene expression , ribosome , rna

The low-copy-number 8.7-kb plasmid pGIF3 of Streptomyces incarnatus was studied after cloning in the Escherichia coli vector pBR322; a restriction map was constructed. Southern blot analysis showed that pGIF3 in S. incarnatus occurs predominantly as integrated in a larger replicon. The plasmid carries a gene for thiostrepton resistance having no homology with the known thiostrepton resistance gene from Streptomyces azureus.

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