Open AccessTransfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression
Author(s) -
Catherine Bourgouin,
A Del�cluse,
Françoise de la Torre,
Jekisiel Szulmajster
Publication year - 1990
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.56.2.340-344.1990
Subject(s) - bacillus thuringiensis , bacillus sphaericus , biology , anopheles stephensi , microbiology and biotechnology , toxin , bacillaceae , aedes aegypti , bacillales , culex pipiens , bacillus (shape) , gene , bacteria , botany , genetics , larva , bacillus subtilis
The genes encoding the toxic determinants of Bacillus sphaericus have been expressed in a nontoxic and a toxic strain of Bacillus thuringiensis subsp. israelensis. In both cases, the B. sphaericus toxin proteins were produced at a high level during sporulation of B. thuringiensis and accumulated as crystalline structures. B. thuringiensis transformants expressing B. sphaericus and B. thuringiensis subsp. israelensis toxins did not show a significant enhancement of toxicity against Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae.
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