Evaluation of methods for enumeration of Vibrio parahaemolyticus from seafood | Zendy

I. Karunasagar | Zendy; M. N. Venugopal | Zendy; K. Segar | Zendy

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Evaluation of methods for enumeration of Vibrio parahaemolyticus from seafood

Author(s) -

I. Karunasagar,

M. N. Venugopal,

K. Segar

Publication year - 1986

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.52.3.583-585.1986

Subject(s) - vibrio parahaemolyticus , enumeration , sodium thiosulfate , thiosulfate , agar , vibrionaceae , microbiology and biotechnology , polymyxin , most probable number , sucrose , food science , vibrio , biology , chemistry , chromatography , bacteria , antibiotics , mathematics , inorganic chemistry , sulfur , genetics , organic chemistry , combinatorics

The efficiency of several enrichment broths in recovering Vibrio parahaemolyticus inoculated into fish homogenates was studied. Recovery by the most probable number technique was very low in all the broths, while direct plating on thiosulfate citrate bile salt sucrose agar yielded better recovery. A decrease in the enrichment time to 8 from 18 h did not improve recovery. At concentrations exceeding 2.5 micrograms/ml, polymyxin was inhibitory to V. parahaemolyticus.

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