Detection of Giardia lamblia by immunofluorescence
Author(s) -
John L. Riggs,
Kent Dupuis,
Keita Nakamura,
D P Spath
Publication year - 1983
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.45.2.698-700.1983
Subject(s) - giardia lamblia , fluorescein isothiocyanate , fluorescein , immunofluorescence , biology , giardia , protozoa , microbiology and biotechnology , titer , fluorescence , antibody , immunology , physics , quantum mechanics
High-titer immune sera to cysts of Giardia lamblia, produced in guinea pigs, were labeled with fluorescein isothiocyanate. The resulting conjugates were used to detect G. lamblia in stool specimens by fluorescence microscopy. The sera also reacted with cysts of Chilomastix mesnili, but the two organisms could be differentiated by their size.
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