Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses | Zendy

Gérald Goldstein | Zendy; Louis E. Guskey | Zendy

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Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses

Author(s) -

Gérald Goldstein,

Louis E. Guskey

Publication year - 1982

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.44.2.317-320.1982

Subject(s) - shaker , echovirus , biology , poliovirus , coxsackievirus , cell culture , enterovirus , virology , kidney , tissue culture , african green monkey , microbiology and biotechnology , virus , in vitro , biochemistry , genetics , physics , quantum mechanics , vibration

Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.

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