Improved growth medium for Campylobacter species
Author(s) -
Ira J Mehlman,
Aida Romero
Publication year - 1982
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.43.3.615-618.1982
Subject(s) - yeast extract , campylobacter , agar , bacteria , salt (chemistry) , food science , agar plate , yeast , starch , microbiology and biotechnology , chemistry , biology , nitrogen , growth medium , fermentation , biochemistry , organic chemistry , genetics
Campylobacter species were grown in a base containing proteose peptone no. 3, yeast extract, K2HPO4, (NH4)2SO4, NA2SO3, soluble starch, and agar. Concentrations and sources of organic nitrogen and growth factors were critical, and the optimal pH range was 7.0 to 7.5. Cultures tolerated 0.7% NaCl in addition to the salt present in the organic constituents and were sensitive to surface-active agents at concentrations recommended for enrichment of other gram-negative bacteria. Cultures were maintained on the proposed medium for 1 year with transfer every 2 weeks.
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