Open AccessDifferential stain for mycobacteria that does not require heat
Author(s) -
Gabriella Tamasi
Publication year - 1981
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.41.1.311-.1981
Subject(s) - differential staining , stain , bacteria , toluidine , microbiology and biotechnology , triethylene glycol , chemistry , aqueous solution , acid fast , mycobacterium , chromatography , staining , biology , organic chemistry , pathology , medicine , tuberculosis , genetics , sputum
A stain of 0.1% toluidine blue in a 1.0% aqueous solution of triethylene glycol followed by decolorization with acid-alcohol resulted in mycobacteria retaining the stain (violet), whereas non-acid-fast bacteria were decolorized.
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