Multichannel photometer-nephelometer
Author(s) -
F. Keilmann,
D. Böhme,
L. Santo
Publication year - 1980
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.40.3.458-461.1980
Subject(s) - nephelometer , photometer , optics , wavelength , light intensity , laser , intensity (physics) , range (aeronautics) , thermostat , laser light , materials science , light scattering , physics , remote sensing , scattering , geology , composite material , thermodynamics
We describe an instrument for monitoring either transmitted or scattered light intensity, or both, simultaneously on up to eight channels. The use of a laser light source (at 632.8-nm wavelength) provides high accuracy and dynamic range: optical density can be measured from 0.0004 up to 6, and a scattered light fraction down to 10(-6) can be resolved. Built-in thermostat and magnetic stirrers allow precise monitoring of aqueous microbial growth over a practical range of 4 orders of magnitude of cell concentration.
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