Determination of lipopolysaccharide by a bioluminescence technique | Zendy

S. Ulitzur | Zendy; B. Yagen | Zendy; Shlomo Rottem | Zendy

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Determination of lipopolysaccharide by a bioluminescence technique

Author(s) -

S. Ulitzur,

B. Yagen,

Shlomo Rottem

Publication year - 1979

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.37.4.782-784.1979

Subject(s) - myristic acid , lipopolysaccharide , bioluminescence , bacteria , bioassay , hydrolysate , chemistry , lipid a , chromatography , biochemistry , microbiology and biotechnology , biology , fatty acid , palmitic acid , hydrolysis , genetics , endocrinology

The determination of the lipid A content of bacterial lipopolysaccharide by using a dim mutant of the luminous bacterium Beneckea harveyi is described. The luminous bacteria emitted light upon the addition of an acid hydrolysate of lipopolysaccharide which contained myristic acid, thus making it possible to detect as little as 1 ng of lipopolysaccharide. By converting the 3-OH-myristic acid to myristic acid, it was possible to further increase the detection sensitivity and to establish a basis for a specific and highly sensitive bioassay for the detection of lipopolysaccharide.

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