Open AccessInclusion of xylan in a medium for the enumeration of total culturable rumen bacteria
Author(s) -
Penelope A. Henning,
A E van der Walt
Publication year - 1978
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.35.6.1008-1011.1978
Subject(s) - rumen , hemicellulose , cellulose , enumeration , xylan , starch , food science , cellobiose , bacteria , biology , fiber , polysaccharide , chemistry , botany , biochemistry , fermentation , cellulase , mathematics , organic chemistry , genetics , combinatorics
The influence of the inclusion of xylan in a medium for enumeration of total culturable rumen bacteria was investigated. Maximum colony numbers were obtained on a medium, GCSX-2, which contained 0.033% each glucose and cellobiose and 0.067% each soluble starch and xylan. This medium gave higher colony counts than either medium 98-5 of Bryant and Robinson (J. Dairy Sci. 44:1446-1456, 1961), medium 98-5 of Chung and Hungate (Appl. Environ. Microbiol. 32:649-652, 1976), containing an added lucerne (hemicellulose + cellulose) fiber substrate, or medium GCSX-2 with the added lucerne (hemicellulose + cellulose) fraction. The time of collection of rumen fluid influenced the colony counts on the media containing the lucerne fiber substrate but was without effect on medium GCSX-2.
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