Control of nonspecific staining in the fluorescent antibody technique for the detection of salmonellae in foods | Zendy

B. Swaminathan | Zendy; John C. Ayres | Zendy; James E. Williams | Zendy

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Control of nonspecific staining in the fluorescent antibody technique for the detection of salmonellae in foods

Author(s) -

B. Swaminathan,

John C. Ayres,

James E. Williams

Publication year - 1978

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.35.5.911-919.1978

Subject(s) - antiserum , conjugate , staining , antibody , salmonella , affinity chromatography , chromatography , globulin , chemistry , staphylococcus aureus , fluorescence , biology , biochemistry , microbiology and biotechnology , bacteria , enzyme , immunology , mathematical analysis , genetics , physics , mathematics , quantum mechanics

A fluorescent antibody conjugate, prepared from the IgG (immunoglobulin G) fraction of Salmonella polyvalent flagellar antiserum, gave better specific staining intensities and significantly lower nonspecific staining than did conjugates prepared from globulin fractions of ammonium sulfate-fractionated Salmonella polyvalent antisera. IgG was purified by affinity chromatography against protein A, a normal cell wall component of Staphylococcus aureus. Affinity chromatography yielded high-purity IgG in a one-step purification procedure. The conjugate prepared from affinity-purified IgG was compared with commercially available fluorescent antibody conjugates for the detection of salmoneallae in retail samplings of meats and poultry and gave better correlations with the cultural method than did the commercial conjugates.

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