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Production of Venezuelan equine encephalitis virus in cells grown on artificial capillaries
Author(s) -
Alexandra Johnson,
Gerald A. Eddy,
J. David Gangemi,
Helen H. Ramsburg,
Julia Metzger
Publication year - 1978
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.35.2.431-434.1978
Subject(s) - virology , virus , cell culture , biology , titer , encephalitis , viral replication , extracellular , venezuelan equine encephalitis virus , cell , microbiology and biotechnology , biochemistry , genetics
Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were relatively free of cell debris.

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